Protein complexes are cellular machines that manage and perform most functions in our cells and in all living organisms. Scientists continue to struggle to understand their composition, their structure and how they can malfunction. Because the most revealing and accurate approach—examining native complexes as entire units—has seemed virtually impossible, most analyses have used mass spectrometry of protein fragments, which may lead to partial or misleading results. A team from Northwestern University, in collaboration with Thermo Fisher Scientific, plans to overcome this major barrier in disease research by developing a new kind of mass spectrometer that combines the advantages of Time-of-Flight (TOF) and Fourier Transform (FT) analyzers. This instrument will be used to separate an intact protein complex from a mixture and then detect it directly or activate to release its subunits. The instrument will then detect the intact masses of subunits and the fragmentation products that result from their stepwise disassembly. To this platform, they will couple new separation strategies and software, followed by application of the combined system to mitochondrial complexes isolated from models of aging and kidney cancer. This integrated workflow will constitute a major advance in protein mass spectrometry, accelerate the understanding of disease at a molecular level and address a key challenge of this century: to define the human proteome.
We are members of the The Neuroproteomics and Neurometabolomics Center on Cell-Cell Signaling at the University of Illinois at Urbana-Champaign. If you have a project which relates to the study of addiction mechanisms in the central nervous system, please contact the center at the link below. The Center is built around the overarching theme of cell to cell signaling, integrating research groups with expertise in the fields of analytical chemistry and bioinformatics with those in biological and behavioral neuroscience in a unified, directed approach to discovery of the intricacies of intercellular signaling.
Visit the website: The Neuroproteomics and Neurometabolomics Center on Cell-Cell Signaling.