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BioID

BioID

The biotin ligase, BirA* is fused to a target of interest, cells can then be treated with biotin and targeted proteins can be identified using standard "biotin-avidin" capture techniques.

Related Publications

Roux KJ, Kim DI, Burke B "BioID: a screen for protein-protein interactions." Curr Protoc Protein Sci, 2013, 74() p. Unit 19.23..
Summary | Full Text (DOI)

Kim DI, Birendra KC, Zhu W, Motamedchaboki K, Doye V, Roux KJ "Probing nuclear pore complex architecture with proximity-dependent biotinylation." Proc Natl Acad Sci U S A, 2014, 111(24) p. E2453-61.
Summary | Full Text (PMC) | Full Text (DOI)

Pricing

  • Northwestern/CBC: $150-$200 (depending on complexity)
    External Academic/Nonprofit: $300
    External For-Profit Industry: $450

Protocols

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