Top Down Mass Spectrometry
Top Down Mass Spectrometry is a technique to characterize the entire sequence of a protein with its related modifications. This service line is tailored to the comprehensive analysis of semi-pure proteins with minimal non-target protein contamination. Examples of good candidates for this approach include recombinantly-expressed proteins, in vitro enzyme assay experiments and the characterization of bioactive peptides.
Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC "Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation." ACS Synth Biol, 2014, 3(6) p. 398-409.
Summary | Full Text (PMC) | Full Text (DOI)
- Northwestern/CBC: $184-$294 (depending on number of samples submitted, and length of LCMS gradient)
LCC: $184-$294 (depending on number of samples submitted, and length of LCMS gradient)
External Academic/Nonprofit: $276-$442
External For-Profit Industry: $460-$736