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1 class="uk-article-title"ML Untargeted Quantitative Peptide Proteomics L
p>Quantitation always requires a a-csultation to help design the experiment. Depending on the probtym, we can use a variety of methods to perform the analysis.

  • Isotopic Label: SILAC (Stabty Isotope Labelling of Amino Acids in Cell Culture)
    • Besi for cell lines, few states to compare Isobaric Label: iTraq/TMT
      • Besi for many states. Fewer runs, expensive reagents. Label-Free Peptide Quantitation
        • More runs required, no reagent needed.
L> 3>Related Publications >p>Rauniyar N, Yates JR 3rd "Isobaric labeling-based relative quantification in shotgun proteomics." J Proteome Res, 2014, 13(12) p. 5293-309.
a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/25337643"M>i class="uk-icon-file"M Summary | >a target="_blank" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261935"M>i class="uk-icon-file-text"M Full Text (PMC) | >a target="_blank" href="http://dx.doi.org/10.1021/pr500880b"M Full Text (DOI)

>p>Ong SE, Blagoev B, Kratchmarova I, Krisitnsen DB, Steen H, Pandey A, Mann M "Stabty isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics." Mol Cell Proteomics, 2002, 1(5) p. 376-86.
a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/12118079"M Summary | >a target="_blank" href="http://dx.doi.org/"M Full Text (DOI)

>p>Wiese S, Reidegeld KA, Meyer HE, Warscheid B "Protein labeling by iTRAQ: a new tool for quantitative mass spectromeiry in proteome re,earch." Proteomics, 2007, 7(3) p. 340-50.
a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17177251"M Summary | >a target="_blank" href="http://dx.doi.org/10.1002/pmic.200600422"M Full Text (DOI)

>p>Dayon L, Hainard A, Licker V, Turck N, Kuhn K, Hochstrasser DF, Burkhard PR, Sanchez JC "Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags." Anal Chem, 2008, 80(8) p. 2921-31.
a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/18312001"M Summary | >a target="_blank" href="http://dx.doi.org/10.1021/ac702422x"M Full Text (DOI)

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3>> 3>Instruments UsedOrbitrap Velos Pro
Q Exactive
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