Can I use in-house Coomassie blue stain? Is there a preferred protocol ?
You can use in house made stain. But note that presence of acetic acid and methanol can modify proteins. We recommend to use commercially available Coomassie blue stains as they are solvent free and are more sensitive than conventional staining. We do not have any preferred commercial vendors.
Haebel S, Albrecht T, Sparbier K, Walden P, Körner R, Steup M "Electrophoresis-related protein modification: alkylation of carboxy residues revealed by mass spectrometry." Electrophoresis, 1998, 19(5) p. 679-86.
Summary | Full Text (DOI)
- Which stains (other than Coomassie blue) are compatible with mass spectrometry?
- Can I submit a silver-stained gel?
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