I have detergent in my sample. Are there ways to remove it?
Yes. The simplest and cheapest way is to use precipitation technique like TCA and acetone precipitation. The downside of these methods is that once precipitated, some proteins are hard to resuspend in the buffer of choice. At Northwestern Proteomics, we offer a service to remove the detergent using Pierce detergent removal kit. We have successfully used this kit for removal of octyl maltosides and SDS from the sample. It should be noted that success of all any technique will depend on concentration and type of the detergent.
Posted in Sample Preparation